The HARPOON kit is assembled and packaged by Dr. Jenny Schuttlefield-Christus’ group at UW Oshkosh. Kits are shipped out once payment is received. The HARPOON kit costs $120 + plus shipping costs. See below for materials included with kit purchase. If you would like to order a kit, please email us through the contact page and include both a billing and shipping address for us to include on an invoice.

These materials are provided with the purchase of a kit ($120):

dish

24 oz Salad dish from Dart Solo


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FTO‐coated glass slides, 3” x 3” square (Additional pieces available from Hartford Glass Co. Phone: 765-348-1282. Ask for 3 x 3″  TEC15 glass).


battery

3V Battery power supply (2 AA batteries required)

lid

Salad dish lid from Dart Solo


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Fluorescent mesh screen (Mesh from Grainger part #3DLP3, paint from Innovative Scientific Solutions Incorporated- Binary UniCoat Pressure Sensitive Paint)


alligator clip

2 Alligator clip test leads

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 Graphite rod (Available from Wale Apparatus, part 12-1201, 1/8” diameter x 12” length)


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Acrylic electrode holder


flashlight

2  UV (395nm) LED flashlights by Esco-Lite (requires 3 AA batteries)

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Copper tape with conducting adhesive (Available from McMaster-Carr, part 76555A712, 1/2″ wide)


rubber bands

2 Large rubber bands (5 x 5/8″)



Additional items required not provided with the kit:

sc2_kiln

Kiln that can hold 500 degrees C for 3 hours (Small jewelry kilns like Paragon SC2 tend to be cheap and work well. Often the school art department has a kiln the group can borrow as well)


Micro_Pipette

Micropipette (5-25 μL range) and disposable tips (Cheap version with 25 μL range)

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Cylinder of inert gas (nitrogen or argon) with hose


copier_paper

Printer paper box or banker’s box

Monitor, Keyboard, and Mouse

Computer (either Windows or Mac)


camera-app-note-3

Camera phone (other digital cameras work too)

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Sodium hydroxide, 0.1 M aqueous solution (approximately 200mL required per experimental run)


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Isopropanol or acetone for cleaning the FTO glass plates before spotting


The assembled HARPOON kit:

dish set up

The dish setup goes inside of the box. The UV flashlights in the side of the box will shine upon the glass plate and mesh screen.  The illuminated mesh screen should be viewable through a filtered hole in the top of the box. Every 30 seconds for 10 minutes, a photo of the screen will be taken with a cellphone camera positioned on top of the hole.

 


Data processing:

Students will  upload their photos to a computer and use the public-domain image processing software, ImageJ, to process the data. The image processing steps have been mostly automated by the development of Java code macros. In the final step, assay activities are mapped onto compositional diagrams to reveal promising “hits.”

data processing